The primary objective of this protocol is to collect and store peripheral blood samples from different acute and chronic, myeloid and lymphoid leukemias and non-leukemia cancer patients for research analysis. Freshly collected blood leukocytes will be separated from red blood cells on a ficoll gradient, counted, and stored in 5-10x10E6 aliquots. RNA will be extracted from one 5x106 aliquot. We will thaw and test 5-sample batches by flow cytometry for the cell-surface expression of previously described (for example CD33, CD123, CD34) and novel antigens (to be determined). Immune cells isolated from patient blood will be studied using multi-parameter flow cytometry +/- cell culture techniques and followed with functional assays (such as phagocytosis, killing, proliferation, and cytokine production) in the presence of autologous or allogeneic tumor cells. None
